《植物生理学报》 2009, 45(3): 223-228

水稻基部伸长节间性状与茎秆机械强度的相关分析和QTL 定位

张喜娟, 姜树坤, 郑旭, 徐正进*, 陈温福, 马殿荣, 徐海

沈阳农业大学, 辽宁省北方粳稻育种重点开放实验室, 农业部作物生理生态遗传育种重点开放实验室, 沈阳110161

通信作者：徐正进；E-mail: xuzhengjin@126.com；Tel: 024-88487183

摘　要：

分析水稻品种 ‘ 沈农 265’ 和 ‘ 丽江新团黑谷 ’ 杂交的 F2 群体基部第二节间茎秆机械强度与该节间形态和茎秆解剖结 构的相关性, 并对基部第二节间机械强度和相关性状进行 QTL 定位的结果表明: 机械强度与茎粗、茎壁面积、茎壁厚度、 大小维管束数目、大小维管束面积、大小维管束韧皮部面积、大小维管束木质部面积呈显著或极显著的正相关, 与节间长 度呈极显著的负相关, 而与茎秆扁平率的相关不显著。采用复合区间作图, 从研究的14 个目标性状中检测到18 个QTL。控 制基部第二节间的抗折力的 QTL 检测到 4 个, 位于第 4、7、9 和 10 号染色体上, 可解释遗传变异的 12%~23%。在第 4 和 第 7 染色体上的相同区间上还同时检测到了控制茎壁性状和维管束性状 QTL, 贡献率在12%~21% 之间。说明这两个位点 是控制基部第二节间机械强度的重要区域, 也是茎壁性状、维管束性状与机械强度高度正相关的遗传学基础。

关键词：水稻; 茎秆机械强度; 抗倒伏; 茎秆形态; 茎秆解剖结构

收稿：2008-09-11   修定：2009-02-02

资助：辽宁省高等学校优秀人才支持计划(2006R48)。

Correlation between Traits of Basal Elongating Internodes and Culm Mechanical Strength and QTL Location in Rice

ZHANG Xi-Juan, JIANG Shu-Kun, ZHENG Xu, XU Zheng-Jin*, CHEN Wen-Fu, MA Dian-Rong, XU Hai

Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Key Laboratory of Northern Japonica

Corresponding author: XU Zheng-Jin; E-mail: xuzhengjin@126.com; Tel: 024-88487183

Abstract:

The correlation between the culm mechanical strength of basal second internode and related morphological and anatomical traits were analyzed in an F₂ population, which was derived from a cross between super japonica rice ‘Shennong265’ and japonica rice cultivar ‘Lijiangxintuanheigu’. And QTLs detection of these traits were employed in this population. The conclusion showed that culm strength was positively correlated with stem diameter, area of culm wall, wall thickness and vascular bundle characters, but negatively correlated with internodes length, and no significant correlation to oblate rate. A total of 18 QTLs for 14 traits were detected by composite interval analysis, including 4 QTLs for culm strength of the basal second internode on chromosome 4, 7, 9, and 10. The variances explained by each QTL from 12% to 23%. The QTLs controlled some culm and vascular bundle traits were detected on the same area on chromosome 4 and 7. The phenotypic variations (VE) explained by individual QTL ranged from 12% to 21%. All the information suggests that these QTLs are important for the culm strength of the basal second internode, and are the important genetic basis of the positive correlation between culm, vascular bundle and stem strength traits.

Key words: rice; culm mechanical strength; lodging resistance; culm configuration; culm anatomical trait